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| Registros recuperados : 17 | |
| 5. |  | SILVA, P. R. A. da; DRESCHEL, M. M.; SOARES, C. de P.; VIDAL, M. S.; BALDANI, J. I. Efeito antagônico de bactérias diazotróficas contra fungos patógenos de cana-de-açúcar In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 13., 2013, Seropédica Ciência, saúde e esporte: caderno de resumos... Seropédica: Embrapa Agrobiologia, 2013.| Biblioteca(s): Embrapa Agrobiologia. |
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| 7. | | SILVA, P. R. A. da; SCHWAB, S.; ARAUJO, J. L. S. de; TEIXEIRA, K. R. dos S.; VIDAL, M. S.; BALDANI, J. I. Genômica funcional da bactéria diazotrófica Burkholderia tropica cultivada em líquido de apoplasto de cana-de-açúcar In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 12., 2012. Economia verde, sustentabilidade e erradicação da pobreza: caderno de resumos. Seropédica: Embrapa Agrobiologia, 2012.| Biblioteca(s): Embrapa Agrobiologia. |
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| 8. | | POLESE, V.; SILVA, P. R. A.; TADRA-SFEIR, M. Z.; ARAUJO, J. L. S. de; SOUZA, E. M.; BALDANI, J. I.; VIDAL, M. S. Functional genomic of Herbaspirillum rubrisubalbicans HCC103 strain grown in the presence of sugarcane apoplastic liquid In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., 2015, Florianópolis. Resumos. Florianópolis: Sociedade Brasileira de Microbiologia, 2015.| Biblioteca(s): Embrapa Agrobiologia. |
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| 9. | | POLESE, V.; SILVA, P. R. A.; VALE, E.; HERINGER, A. S.; SOUZA FILHO, G. A. de; SILVEIRA, V.; BALDANI, J. I.; VIDAL, M. S. The effect of sugarcane apoplastic fluid on the proteome of HCC103 strain of Herbaspirillum rubrisubalbicans. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16., LATINAMERICAN WORKSHOP OF PGPR, 4., RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Emprapa, 2018. p. 43 Session 4, Poster III.9| Biblioteca(s): Embrapa Agrobiologia. |
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| 10. | | SILVA, P. R. A.; ARAUJO, J. L. S. de; VIDAL, M. S.; CRUZ, L. M.; SOUZA, E. M. de; BALDANI, J. I. Draft genome sequence of Paraburkholderia tropica Ppe8 strain, a sugarcane endophytic diazotrophic bacterium. Brazilian Journal of Microbiology, v. 49, n. 2, p. 2010-2011, Apr./Jun. 2018.| Biblioteca(s): Embrapa Agrobiologia. |
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| 11. | | SILVA, P. R. A.; VIDAL, M. S.; POLESE, V.; TADRA-SFEIR, M. Z.; ARAUJO, J. L. S. de; SOUZA, E. M.; BALDANI, J. I. Differential gene expression of diazotrophic burkholderia tropica PPE5 strain cultivated in sugarcane apoplast fluid In: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2015.| Biblioteca(s): Embrapa Agrobiologia. |
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| 12. | | SILVA, P. R. A. da; VIDAL, M. S.; SOARES, C. de P.; POLESE, V.; ARAUJO, J. L. S. de; BALDANI, J. I. Selection and evaluation of reference genes for RT-qPCR expression studies on Burkholderia tropica strain Ppe8, a sugarcane-associated diazotrophic bacterium grown with different carbon sources or sugarcane juice Antonie van Leeuwenhoek, v. 109, n. 111, p. 1493-1502, 2016. Epub, 17 Aug., 2016.| Biblioteca(s): Embrapa Agrobiologia. |
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| 13. | | POLESE, V.; SOARES, C. de P.; SILVA, P. R. A. da; ARAUJO, J. L. S. de; BALDANI, J. I.; VIDAL, M. S. Selection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103. Antonie van Leeuwenhoekv, 110, n. 12, p. 1555-1568, 2017.| Biblioteca(s): Embrapa Agrobiologia. |
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| 14. | | POLESE, V.; SILVA, P. R. A.; PESSOA, D. D. V.; TADRA-SFEIR, M. Z.; SOUZA, E. M.; ARAUJO, J. L. S. de; BALDANI, J. I.; VIDAL, M. S. Transcriptional profile of Herbaspirillum rubrisubalbicans in response of sugarcane apoplastic fluid. In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 11., 2018, Auckland, New Zealand. Proceedings... Massey University, 2018.| Biblioteca(s): Embrapa Agrobiologia. |
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| 15. | | SILVA, P. R. A. da; VIDAL, M. S.; TADRA-SFEIR, M.Z.; ARAUJO, J. L. S. de; SOUZA, E. M. de; BALDANI, J. I. In vitro study suggests that stem apoplastic liquid of sugarcance modulates the chemotaxis and defense mechanism of the diazotrophic Burkholderia tropica strain PPe8 In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION WITH NON-LEGUMES, 15.; SYMPOSIUM OF THE EUROPEAN NITROGEN FIXATION CONFERENCE, 12., 2016, Hungary. Book of abstracts. Hungary: Biological Research Center, 2016. p. 47| Biblioteca(s): Embrapa Agrobiologia. |
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| 16. | | ROCHA, F. Y. O.; OLIEIRA, C. M. de; SILVA, P. R. A. da; MELO, L. H. V. de; CARMO, M. G. F. do; BALDANI, J. I. Taxonomical and functional characterization of Bacillus strains isolated from tomato plants and their biocontrol activity against races 1, 2 and 3 of Fusarium oxysporum f. sp. Lycopersici Applied Soil Ecology, London, v. 120, p. 8-19, 2017| Biblioteca(s): Embrapa Agrobiologia. |
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| 17. | | SILVA, P. R. A. da; VIDAL, M. S.; SOARES, C. de P.; POLESE, V.; TADRA-SFEIR, M. Z.; SOUZA, E. M. de; ARAUJO, J. L. S. de; BALDANI, J. I. Sugarcane apoplast fluid modulates the global transcriptional profile of the diazotrophic bacteria Paraburkholderia tropica strain Ppe8. PLOS ONE, v. 13, n. 12, e0207863, 2018.| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 17 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
24/11/2016 |
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Data da última atualização: |
23/10/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVA, P. R. A. da; VIDAL, M. S.; SOARES, C. de P.; POLESE, V.; ARAUJO, J. L. S. de; BALDANI, J. I. |
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Afiliação: |
PAULA RENATA ALVES DA SILVA, UFRRJ; MARCIA SOARES VIDAL, CNPAB; CLEITON DE PAULA SOARES, BOLSISTA DA EMBRAPA AGROBIOLOGIA; VALERIA POLESE, UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; JOSE IVO BALDANI, CNPAB. |
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Título: |
Selection and evaluation of reference genes for RT-qPCR expression studies on Burkholderia tropica strain Ppe8, a sugarcane-associated diazotrophic bacterium grown with different carbon sources or sugarcane juice |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Antonie van Leeuwenhoek, v. 109, n. 111, p. 1493-1502, 2016. |
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DOI: |
10.1007/s10482-016-0751-0 |
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Idioma: |
Inglês |
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Notas: |
Epub, 17 Aug., 2016. |
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Conteúdo: |
Among the members of the genus Burkholderia, Burkholderia tropica has the ability to fix nitrogen and promote sugarcane plant growth as well as act as a biological control agent. There is little information about how this bacterium metabolizes
carbohydrates as well as those carbon sources found in the sugarcane juice that accumulates in stems during plant growth. Reverse transcription quantitative PCR (RT-qPCR) can be used to evaluate changes in gene expression during bacterial growth on different carbon sources. Here we tested the expression of six reference genes, lpxC, gyrB, recA, rpoA, rpoB, and rpoD, when cells were grown with glucose, fructose, sucrose, mannitol, aconitic acid, and sugarcane juice as carbon sources. The lpxC, gyrB, and recA were selected as the most stable reference genes based on geNorm and NormFinder software analyses. Validation of these three reference genes during strain Ppe8 growth on the same carbon sources showed that genes involved in
glycogen biosynthesis (glgA, glgB, glgC) and trehalose biosynthesis (treY and treZ) were highly expressed when Ppe8 was grown in aconitic acid relative to other carbon sources, while otsA expression (trehalose biosynthesis) was reduced with all carbon
sources. In addition, the expression level of the ORF_6066 (gluconolactonase) gene was reduced on sugarcane juice. The results confirmed the stability of the three selected reference genes (lpxC, gyrB, and recA) during the RT-qPCR and also their robustness
by evaluating the relative expression of genes involved in glycogen and trehalose biosynthesis when strain Ppe8 was grown on different carbon sources and sugarcane juice. MenosAmong the members of the genus Burkholderia, Burkholderia tropica has the ability to fix nitrogen and promote sugarcane plant growth as well as act as a biological control agent. There is little information about how this bacterium metabolizes
carbohydrates as well as those carbon sources found in the sugarcane juice that accumulates in stems during plant growth. Reverse transcription quantitative PCR (RT-qPCR) can be used to evaluate changes in gene expression during bacterial growth on different carbon sources. Here we tested the expression of six reference genes, lpxC, gyrB, recA, rpoA, rpoB, and rpoD, when cells were grown with glucose, fructose, sucrose, mannitol, aconitic acid, and sugarcane juice as carbon sources. The lpxC, gyrB, and recA were selected as the most stable reference genes based on geNorm and NormFinder software analyses. Validation of these three reference genes during strain Ppe8 growth on the same carbon sources showed that genes involved in
glycogen biosynthesis (glgA, glgB, glgC) and trehalose biosynthesis (treY and treZ) were highly expressed when Ppe8 was grown in aconitic acid relative to other carbon sources, while otsA expression (trehalose biosynthesis) was reduced with all carbon
sources. In addition, the expression level of the ORF_6066 (gluconolactonase) gene was reduced on sugarcane juice. The results confirmed the stability of the three selected reference genes (lpxC, gyrB, and recA) during the RT-qPCR and also their robustness
by evalua... Mostrar Tudo |
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Palavras-Chave: |
Carbon metabolism; Diazotrophic bacteria; Normalizing genes; Real time PCR; Relative expression. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
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001 2057134
005 2018-10-23
008 2016 bl --- 0-- u #d
024 7 $a10.1007/s10482-016-0751-0$2DOI
100 1 $aSILVA, P. R. A. da
245 $aSelection and evaluation of reference genes for RT-qPCR expression studies on Burkholderia tropica strain Ppe8, a sugarcane-associated diazotrophic bacterium grown with different carbon sources or sugarcane juice
260 $c2016
500 $aEpub, 17 Aug., 2016.
520 $aAmong the members of the genus Burkholderia, Burkholderia tropica has the ability to fix nitrogen and promote sugarcane plant growth as well as act as a biological control agent. There is little information about how this bacterium metabolizes carbohydrates as well as those carbon sources found in the sugarcane juice that accumulates in stems during plant growth. Reverse transcription quantitative PCR (RT-qPCR) can be used to evaluate changes in gene expression during bacterial growth on different carbon sources. Here we tested the expression of six reference genes, lpxC, gyrB, recA, rpoA, rpoB, and rpoD, when cells were grown with glucose, fructose, sucrose, mannitol, aconitic acid, and sugarcane juice as carbon sources. The lpxC, gyrB, and recA were selected as the most stable reference genes based on geNorm and NormFinder software analyses. Validation of these three reference genes during strain Ppe8 growth on the same carbon sources showed that genes involved in glycogen biosynthesis (glgA, glgB, glgC) and trehalose biosynthesis (treY and treZ) were highly expressed when Ppe8 was grown in aconitic acid relative to other carbon sources, while otsA expression (trehalose biosynthesis) was reduced with all carbon sources. In addition, the expression level of the ORF_6066 (gluconolactonase) gene was reduced on sugarcane juice. The results confirmed the stability of the three selected reference genes (lpxC, gyrB, and recA) during the RT-qPCR and also their robustness by evaluating the relative expression of genes involved in glycogen and trehalose biosynthesis when strain Ppe8 was grown on different carbon sources and sugarcane juice.
653 $aCarbon metabolism
653 $aDiazotrophic bacteria
653 $aNormalizing genes
653 $aReal time PCR
653 $aRelative expression
700 1 $aVIDAL, M. S.
700 1 $aSOARES, C. de P.
700 1 $aPOLESE, V.
700 1 $aARAUJO, J. L. S. de
700 1 $aBALDANI, J. I.
773 $tAntonie van Leeuwenhoek$gv. 109, n. 111, p. 1493-1502, 2016.
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